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Anthocyanin Assay

Anthocyanidin is a class of water-soluble pigment compounds that widely exist in plants. Free anthocyanins are rare under natural conditions and mainly exist in the form of glycosides. Metware's Anthocyanidin Analysis assay is a targeted LC-MS/MS method that will detect and obtain absolute and semi-quantification of 108 compounds in the anthocyanin metabolic pathway.

Technology Introduction

Anthocyanin assay is an anthocyanin targeted metabolomics approach with accurate identification and quantification of 108 anthocyanidins, which include pelargonidins, cyanidins, delphinidins, peonidins, petunidins, malvidins, procyanidins, among others.

Technology Introduction

Technical Features of Anthocyanin Assay Service

Wide Coverage
108 anthocyanidin metabolites over 8 different types.
Absolute Quantification
Absolute anthocyanin quantification of 41 metabolites with standard curver > 0.99 and semi-quantification of 67 metabolites.

Applications of Anthocyanin Assay Service

Applications: Research In Plant Color
Applications: Research In Stress Response And Environmental Adaptation

Abiotic stress: light (UV), water (drought, waterlogging), heavy metals (cadmium, lead, etc.), temperature (high temperature,low temperature), mechanical damage and other external factors bring reversible or irreversible effects on plants.


Biological stress: the influence of insects, bacteria, fungi, and viruses on the growth and development of plants.


Article Spotlight: Transcriptomics integrated with metabolomics reveals the effect of regulated deficit irrigation on anthocyanin biosynthesis in Cabernet Sauvignon grape berries.


Regulated deficit irrigation (RDI) is a new type of water-saving irrigation technology developed in recent years which was well suited to arid and semi-arid grape plant areas. The anthocyanin synthesis of grapes under RDI was revealed through omics in this study. RDI slightly decreased the hundred-grain weight and increased the soluble solid content, juice pH, reducing sugar content, and total anthocyanin content. Meanwhile, the total acid content decreased before ripening. Transcriptomics and metabolomics analyses revealed that large numbers of differentially expressed genes (DEGs) and significantly changed metabolites (SCMs) were filtered in the RDI groups. RDI1 with 30% ETc upregulated 7 related gene expression levels in the anthocyanin biosynthetic pathway and also increased some metabolites contents. Eventually, the contents of most monomeric antho cyanins in the RDI groups were increased, and the proportion of Mv increased in the ripe grapes of the RDI groups. In all, RDI is a useful water-saving irrigation method that could also increase anthocyanin content in grapes.


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List of Metabolites

Anthocyanin

database

v2.0

 

NO.

Compounds

Class

Quantitative

1

Cyanidin-3-(6''-caffeylsophoroside)-5-glucoside

Cyanidin

Semiquantitative

2

Cyanidin-3-(6-O-p-caffeoyl)-glucoside

Cyanidin

Semiquantitative

3

Cyanidin-3,5,3'-O-triglucoside

Cyanidin

Semiquantitative

4

Cyanidin-3,5-O-diglucoside

Cyanidin

Semiquantitative

5

Cyanidin-3-O-(6''-ferulylsophoroside)-5-glucoside

Cyanidin

Semiquantitative

6

Cyanidin-3-O-(6-O-malonyl-beta-D-glucoside)

Cyanidin

Semiquantitative

7

Cyanidin-3-O-5-O-(6-O-coumaroyl)-diglucoside

Cyanidin

Semiquantitative

8

Cyanidin-3-O-galactoside

Cyanidin

Semiquantitative

9

Cyanidin-3-O-glucoside

Cyanidin

Semiquantitative

10

Cyanidin-3-O-rutinoside

Cyanidin

Semiquantitative

11

Cyanidin-3-O-rutinoside-5-O-glucoside

Cyanidin

Semiquantitative

12

Cyanidin-3-O-sambubioside-5-O-glucoside

Cyanidin

Semiquantitative

13

Cyanidin-3-O-sophoroside

Cyanidin

Semiquantitative

14

Cyanidin-3-O-(6-O-p-coumaroyl)-glucoside

Cyanidin

Absolute quantification

15

Cyanidin-3-O-arabinoside

Cyanidin

Absolute quantification

16

Cyanidin-3-O-sambubioside

Cyanidin

Absolute quantification

17

Cyanidin-3-O-xyloside

Cyanidin

Absolute quantification

18

Delphinidin-3-O-(6-O-acetyl)-glucoside

Delphinidin

Semiquantitative

19

Delphinidin-3-O-(6-O-malonyl)-glucoside-3'-glucoside

Delphinidin

Semiquantitative

20

Delphinidin-3-O-rhamnoside

Delphinidin

Semiquantitative

21

Delphinidin-3-O-rutinoside

Delphinidin

Semiquantitative

22

Delphinidin-3-O-rutinoside-5-O-glucoside

Delphinidin

Semiquantitative

23

Delphinidin-3-O-sambubioside

Delphinidin

Semiquantitative

24

Delphinidin-3-O-sambubioside-5-O-glucoside

Delphinidin

Semiquantitative

25

Delphinidin-3-O-sophoroside

Delphinidin

Semiquantitative

26

Delphinidin-3,5-O-diglucoside

Delphinidin

Absolute quantification

27

Delphinidin-3-O-(6-O-malonyl-beta-D-glucoside)

Delphinidin

Absolute quantification

28

Delphinidin-3-O-(6-O-p-coumaroyl)-glucoside

Delphinidin

Absolute quantification

29

Delphinidin-3-O-5-O-(6-O-coumaroyl)-diglucoside

Delphinidin

Absolute quantification

30

Delphinidin-3-O-arabinoside

Delphinidin

Absolute quantification

31

Delphinidin-3-O-galactoside

Delphinidin

Absolute quantification

32

Delphinidin-3-O-glucoside

Delphinidin

Absolute quantification

33

Delphinidin

Delphinidins

Absolute quantification

34

Kaempferol-3-O-rutinoside

flavonoid

Semiquantitative

35

Quercetin-3-O-glucoside

flavonoid

Semiquantitative

36

Afzelin

flavonoid

Absolute quantification

37

Chalcone

flavonoid

Absolute quantification

38

Dihydrokaempferol

flavonoid

Absolute quantification

39

Dihydromyricetin

flavonoid

Absolute quantification

40

Naringenin

flavonoid

Absolute quantification

41

Naringenin-7-O-glucoside

flavonoid

Absolute quantification

42

Rutin

flavonoid

Absolute quantification

43

Malvidin-3-O-(6-O-p-coumaroyl)-glucoside

Malvidin

Semiquantitative

44

Malvidin-3-O-5-O-(6-O-coumaroyl)-diglucoside

Malvidin

Semiquantitative

45

Malvidin-3-O-arabinoside

Malvidin

Semiquantitative

46

Malvidin-3-O-galactoside

Malvidin

Semiquantitative

47

Malvidin-3-O-sambubioside-5-O-glucoside

Malvidin

Semiquantitative

48

Malvidin-3-O-sophoroside

Malvidin

Semiquantitative

49

Malvidin

Malvidin

Absolute quantification

50

Malvidin-3,5-O-diglucoside

Malvidin

Absolute quantification

51

Malvidin-3-O-(6''-acetylglucoside)-5-glucoside

Malvidin

Absolute quantification

52

Malvidin-3-O-(6-O-malonyl-beta-D-glucoside)

Malvidin

Absolute quantification

53

Malvidin-3-O-glucoside

Malvidin

Absolute quantification

54

Malvidin-3-O-rutinoside

Malvidin

Absolute quantification

55

Malvidin-3-O-sambubioside

Malvidin

Absolute quantification

56

Pelargonidin

Pelargonidin

Semiquantitative

57

Pelargonidin-3-(6''-caffeylsophoroside)-5-glucoside

Pelargonidin

Semiquantitative

58

Pelargonidin-3,5-O-diglucoside

Pelargonidin

Semiquantitative

59

Pelargonidin-3-O-(6''-ferulylsambubioside)-5-O-(malonyl)-glucoside

Pelargonidin

Semiquantitative

60

Pelargonidin-3-O-(6-O-malonyl-beta-D-glucoside)

Pelargonidin

Semiquantitative

61

Pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside

Pelargonidin

Semiquantitative

62

Pelargonidin-3-O-[2-O-glucosyl-6-O-p-coumaroyl-glucoside]-5-O-glucoside

Pelargonidin

Semiquantitative

63

Pelargonidin-3-O-[6-O-feruloyl-2-O-glucosyl-glucoside]-5-O-glucoside

Pelargonidin

Semiquantitative

64

Pelargonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside

Pelargonidin

Semiquantitative

65

Pelargonidin-3-O-arabinoside

Pelargonidin

Semiquantitative

66

Pelargonidin-3-O-galactoside

Pelargonidin

Semiquantitative

67

Pelargonidin-3-O-glucoside

Pelargonidin

Semiquantitative

68

Pelargonidin-3-O-rutinoside-5-O-glucoside

Pelargonidin

Semiquantitative

69

Pelargonidin-3-O-sambubioside

Pelargonidin

Semiquantitative

70

Pelargonidin-3-O-sambubioside-5-O-glucoside

Pelargonidin

Semiquantitative

71

Pelargonidin-3-O-sophoroside

Pelargonidin

Semiquantitative

72

Pelargonidin-3-O-rutinoside

Pelargonidin

Absolute quantification

73

Pelargonidin-3-O-sophoroside-5-O-(malonyl)-glucoside

Pelargonidin

Absolute quantification

74

Pelargonidin-3-sophoroside-5-glucoside

Pelargonidin

Absolute quantification

75

Peonidin

Peonidin

Semiquantitative

76

Peonidin-3-(caffeoyl-glucosyl-glucoside)-5-glucoside

Peonidin

Semiquantitative

77

Peonidin-3-O-(6-O-p-coumaroyl)-glucoside

Peonidin

Semiquantitative

78

Peonidin-3-O-arabinoside

Peonidin

Semiquantitative

79

Peonidin-3-O-caffeoyl-feruloyl-sophoroside-5-glucoside

Peonidin

Semiquantitative

80

Peonidin-3-O-galactoside

Peonidin

Semiquantitative

81

Peonidin-3-O-glucoside

Peonidin

Semiquantitative

82

Peonidin-3-O-P-hydroxybenzoylsophoroside-5-glucoside

Peonidin

Semiquantitative

83

Peonidin-3-O-rutinoside

Peonidin

Semiquantitative

84

Peonidin-3-O-sambubioside

Peonidin

Semiquantitative

85

Peonidin-3-O-sambubioside-5-O-glucoside

Peonidin

Semiquantitative

86

Peonidin-3-sophoroside-5-glucoside

Peonidin

Semiquantitative

87

Peonidin-3,5-O-diglucoside

Peonidin

Absolute quantification

88

Peonidin-3-O-(6''-ferulylsophoroside)-5-glucoside

Peonidin

Absolute quantification

89

Peonidin-3-O-(6-O-malonyl-beta-D-glucoside)

Peonidin

Absolute quantification

90

Peonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside

Peonidin

Absolute quantification

91

Peonidin-3-O-sophoroside

Peonidin

Absolute quantification

92

Petunidin-3-O-(6-O-p-coumaroyl)-glucoside

Petunidin

Semiquantitative

93

Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside

Petunidin

Semiquantitative

94

Petunidin-3-O-arabinoside

Petunidin

Semiquantitative

95

Petunidin-3-O-glucoside

Petunidin

Semiquantitative

96

Petunidin-3-O-sambubioside

Petunidin

Semiquantitative

97

Petunidin-3,5-O-diglucoside

Petunidin

Absolute quantification

98

Petunidin-3-O-(6-O-malonyl-beta-D-glucoside)

Petunidin

Absolute quantification

99

Petunidin-3-O-galactoside

Petunidin

Absolute quantification

100

Petunidin-3-O-rutinoside

Petunidin

Absolute quantification

101

Petunidin-3-O-sambubioside-5-O-glucoside

Petunidin

Absolute quantification

102

Petunidin-3-O-sophoroside

Petunidin

Absolute quantification

103

Procyanidin B3

Procyanidin

Semiquantitative

104

Procyanidin A1

Procyanidin

Absolute quantification

105

Procyanidin A2

Procyanidin

Absolute quantification

106

Procyanidin B1

Procyanidin

Absolute quantification

107

Procyanidin B2

Procyanidin

Absolute quantification

108

Procyanidin C1

Procyanidin

Absolute quantification

 

Project Workflow of Anthocyanin Assay Service

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