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High Throughput
4000+ lipids can be detected at one time.
Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with absolute quantification of over 4000 lipids.
Lipidomics refers to the study of the structure, function, content changes, and interactions of lipids in organisms to reveal the regulatory role of lipid metabolism on the physiological and pathological state of the organism. Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with absolute quantification of over 4000 lipids. Metware Biotechnology has developed a professional method for the detection of lipids, covering 51 kinds, such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG.
4000+ lipids can be detected at one time.
Simultaneous detection of more than 51 types of lipids such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG, etc.
Better reproducibility of multiple testing results, with intra-batch correction for large sample volumes.
The use of highly sensitive mass spectrometer AB SCIEX6500+, detection down to pg level.
Absolute quantification by internal standard method with more accurate results (standard + isotope internal standard).
Clinical disease research: applied to various types of disease research such as cardiovascular, tumor, immune, endocrine, gastrointestinal, and neurological.
Screening of Biomarkers: by studying the differences in lipid profiles between different groups, screening of Biomarkers can be used for research on disease diagnosis, disease staging/staging, prognosis determination, etc.
Mechanism studies: e.g. mechanism studies of lipid effects on growth, development, energy metabolism, signal transduction processes, and diseases.
Pharmaceutical therapy: e.g. polyketide anti-microbial, anti-parasitic and anti-tumor drugs.
Multi-omics joint research: combined with other histologies to study the physiological and pathological effects of the organism, such as the elucidation of regulatory genes of important lipids that affect disease health and the mechanisms of regulation.
| Number of Lipids | ||
| Class I | Class II | Number |
| Fatty acyls(FA) | CAR, FFA, Eicosanoid, FAHFA | 270 |
| Glycerolipids(GL) | DG, DG-O, MG, TG, TG-O, MGDG, DGDG | 1015 |
| Glycerophospholipids(GP) | LPC, LPC-O, LPE, LPE-P, LPG, LPS, PC, PC-O, PE, PE-P, PE-O, PG, PS, LPI, PI, LPA, PA, PMeOH, BMP, HMBP, LNAPE | 1800 |
| Sphingolipids(SL) | SPH, CerP, HexCer, SM, Cer, Cert | 828 |
| Sterol lipids(ST) | Cho, CE, BA, CASE | 122 |
| Prenol lipids(PR) | CoQ | 3 |
| Total | 4000+ | |
| Sample | Sample Requirements |
| Serum/plasma | 200ul/sample, liquid nitrogen flash frozen and stored at -80℃, sent on dry ice; hemolysis should be avoided as much as possible. |
| Tissue/feces | 200mg/sample, stored at -80℃ after quick-freezing in liquid nitrogen and sent on dry ice. |
| Cells | ≥1×10^7/sample, stored at -80℃ after quick-freezing in liquid nitrogen and sent on dry ice. |
| Stool and intestinal contents | 150mg/sample; stored at -80℃ after quick-freezing in liquid nitrogen and sent on dry ice. |
| Note: For other sample types, please consult the relevant staff; Avoid repeated freezing and thawing | |
Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
