Metware Biotechnology Co., Ltd.
Quantitative Lipidomics

Quantitative Lipidomics

quantitative lipidomics service

Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with absolute quantification of over 4000 lipids.

Technology Introduction

Lipidomics refers to the study of the structure, function, content changes, and interactions of lipids in organisms to reveal the regulatory role of lipid metabolism on the physiological and pathological state of the organism. Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with absolute quantification of over 4000 lipids. Metware Biotechnology has developed a professional method for the detection of lipids, covering 51 kinds, such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG.

untargeted-metabolomics-service-1.png

Technical Features of Quantitative Lipidomics Service

High Throughput

4000+ lipids can be detected at one time.

Wide Coverage

Simultaneous detection of more than 51 types of lipids such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG, etc.

High Reproducibility

Better reproducibility of multiple testing results, with intra-batch correction for large sample volumes.

High Sensitivity

The use of highly sensitive mass spectrometer AB SCIEX6500+, detection down to pg level.

High Accuracy

Absolute quantification by internal standard method with more accurate results (standard + isotope internal standard).

Applications of Quantitative Lipidomics Service

  1. Clinical disease research: applied to various types of disease research such as cardiovascular, tumor, immune, endocrine, gastrointestinal, and neurological.

  2. Screening of Biomarkers: by studying the differences in lipid profiles between different groups, screening of Biomarkers can be used for research on disease diagnosis, disease staging/staging, prognosis determination, etc.

  3. Mechanism studies: e.g. mechanism studies of lipid effects on growth, development, energy metabolism, signal transduction processes, and diseases.

  4. Pharmaceutical therapy: e.g. polyketide anti-microbial, anti-parasitic and anti-tumor drugs.

  5. Multi-omics joint research: combined with other histologies to study the physiological and pathological effects of the organism, such as the elucidation of regulatory genes of important lipids that affect disease health and the mechanisms of regulation.


List of Metabolites

Number of Lipids
Class IClass IINumber
Fatty acyls(FA)CAR, FFA,  Eicosanoid, FAHFA270
Glycerolipids(GL)DG, DG-O, MG, TG, TG-O, MGDG, DGDG1015
Glycerophospholipids(GP)LPC, LPC-O, LPE, LPE-P, LPG, LPS, PC, PC-O, PE, PE-P, PE-O, PG, PS, LPI, PI, LPA, PA, PMeOH, BMP, HMBP, LNAPE1800
Sphingolipids(SL)SPH, CerP, HexCer, SM, Cer, Cert828
Sterol lipids(ST)Cho, CE, BA, CASE122
Prenol lipids(PR)CoQ3
Total4000+


Project Workflow of Quantitative Lipidomics Service

Project Workflow of Quantitative Lipidomics Service

Sample Requirements of Quantitative Lipidomics Service

SampleSample Requirements
Serum/plasma200ul/sample, liquid nitrogen flash frozen and stored at -80℃, sent on dry ice; hemolysis should be avoided as much as possible.
Tissue/feces200mg/sample, stored at -80℃ after quick-freezing in liquid nitrogen and sent on dry ice.
Cells≥1×10^7/sample, stored at -80℃ after quick-freezing in liquid nitrogen and sent on dry ice.
Stool and intestinal contents150mg/sample; stored at -80℃ after quick-freezing in liquid nitrogen and sent on dry ice.
Note: For other sample types, please consult the relevant staff; Avoid repeated freezing and thawing


WHAT'S NEXT IN OMICS: THE METABOLOME
WHAT'S NEXT IN OMICS: THE METABOLOME

Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.