Metware Biotechnology Co., Ltd.
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Quantitative Lipidomics

Quantitative Lipidomics

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Quantitative Lipidomics

Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with semiquantification of over 4000 lipids.

Technology Introduction

Lipidomics refers to the study of the structure, function, content changes, and interactions of lipids in organisms to reveal the regulatory role of lipid metabolism on the physiological and pathological state of the organism. Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with semiquantification of over 4000 lipids. Metware Biotechnology has developed a professional method for the detection of lipids, covering 51 kinds, such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG.

Quantitative-Lipidomics-workflow_2.png

Technical Features of Quantitative Lipidomics Service

High Throughput

4000+ lipids can be detected at one time.

Wide Coverage

Simultaneous detection of more than 51 types of lipids such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG, etc.

High Reproducibility

Better reproducibility of multiple testing results, with intra-batch correction for large sample volumes.

High Sensitivity

The use of highly sensitive mass spectrometer AB SCIEX6500+, detection down to pg level.

High Accuracy

Absolute quantification by internal standard method with more accurate results (standard + isotope internal standard).

Applications of Quantitative Lipidomics Service

  1. Clinical disease research: applied to various types of disease research such as cardiovascular, tumor, immune, endocrine, gastrointestinal, and neurological.

  2. Screening of Biomarkers: by studying the differences in lipid profiles between different groups, screening of Biomarkers can be used for research on disease diagnosis, disease staging/staging, prognosis determination, etc.

  3. Mechanism studies: e.g. mechanism studies of lipid effects on growth, development, energy metabolism, signal transduction processes, and diseases.

  4. Pharmaceutical therapy: e.g. polyketide anti-microbial, anti-parasitic and anti-tumor drugs.

  5. Multi-omics joint research: combined with other histologies to study the physiological and pathological effects of the organism, such as the elucidation of regulatory genes of important lipids that affect disease health and the mechanisms of regulation.



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List of Metabolites

Number of Lipids
Class IClass IINumber
Fatty acyls(FA)CAR, FFA,  Eicosanoid, FAHFA270
Glycerolipids(GL)DG, DG-O, MG, TG, TG-O, MGDG, DGDG1015
Glycerophospholipids(GP)LPC, LPC-O, LPE, LPE-P, LPG, LPS, PC, PC-O, PE, PE-P, PE-O, PG, PS, LPI, PI, LPA, PA, PMeOH, BMP, HMBP, LNAPE1800
Sphingolipids(SL)SPH, CerP, HexCer, SM, Cer, Cert828
Sterol lipids(ST)Cho, CE, BA, CASE122
Prenol lipids(PR)CoQ3
Total4000+


Project Workflow of Quantitative Lipidomics Service

Project Workflow of Quantitative Lipidomics Service

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Sample Requirements of Quantitative Lipidomics Service


Sample type

sample

Recommended sample

Minimum sample

Biological replication

liquid

Plasma, serum, hemolymph, milk, egg white

100 μL

20μL

human>30

animal>8


Cerebrospinal fluid, tear fluid, interstitial fluid, uterine fluid, pancreatic fluid and bile, pleural effusion, follicular fluid, culture medium

100 μL

20μL

Seminal plasma, amniotic fluid, prostate fluid, rumen fluid, respiratory condensate, gastric lavage fluid, alveolar lavage fluid, urine, sweat, corpse fluid, saliva, sputum

500 μL

100 μL

tissue

Animal tissue, placenta, thrombus, fish skin, mycelium, nematode

100 mg

20 mg

Whole body, aircraft (wings), pupae

500 mg

20 mg

Zebrafish organs, insect organs

20

10

cell

Adherent cells

1*10^6

5*10^5

Escherichia coli and other microorganisms

1*10^10

5*10^8

feces

Feces,iIntestinal contents

200 mg 

(Wet weight)

50 mg

(Wet weight)



WHAT'S NEXT IN OMICS: THE METABOLOME
WHAT'S NEXT IN OMICS: THE METABOLOME

Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.