Email:
support-global@metwarebio.com
Apr 23, 2024
Proteomics encompasses various techniques stemming from diverse experimental methods, among which Data-Independent Acquisition (DIA) stands prominent. DIA operates by dynamically setting multiple mass-to-charge ratio windows over time, ensuring the fragmentation and detection of all peptide ions passing through these windows in MS2. This distinctive methodology of DIA excels in discovery proteomics and phenotypic comparisons, facilitating superior peptide identification characterized by heightened precision, stability, and repeatability.
Achieving deeper coverage to mitigate protein loss.
Enhancing accuracy in quantification for more dependable data.
Engaging in precision medicine investigations.
Utilizing MS-based proteomic analysis of blood, urine, and various samples enables the screening of disease biomarkers, facilitating both diagnosis and prognosis.
Functional studies focusing on specific proteins remain foundational in biological research. Through DIA Quantitative Proteomics, exploring individual Protein-Protein Interactions (PPIs) becomes feasible, enhancing our comprehension of human diseases, growth, and development.
DIA Quantitative Proteomics finds application in studying plant development, stress responses, and environmental adaptation.
Sample type | Recommend | Minimum | ||
Animal | Common animal tissues:animal internal organs (heart, liver, spleen, lung, kidney), skin, muscle, brain, etc | ≥5mg | ≥1mg | |
| Mollusks (Toxoplasma, Schistosomiasis, Drosophila, Acarid, Plutella xylostella, Laodelphax, Cestode, Cicada, Hematodinium, etc. | ≥5mg | ≥2mg | ||
Cell | Suspended cells, adherent cells | ≥1×107 | ≥1×106 | |
| Cell culture supernatant | ≥5mL | |||
Exosome | Exosome isolated by customer | ≥20μg, ≥0.5μg/μL | ||
Fluid | Plasma, serum (remove highly-abundant protein) | ≥200 μL | ≥50 μL | |
| Plasma, serum (with highly-abundant protein) | / | / | ||
| Amniotic fluid, cerebrospinal fluid, semen, etc (remove highly-abundant protein) | ≥1 mL | ≥500μL | ||
| Amniotic fluid, cerebrospinal fluid, semen, etc (with highly-abundant protein) | ≥200 μL | ≥100 μL | ||
| Saliva, milk | ≥200 μL | ≥100 μL | ||
| Urine | ≥30 mL | ≥15 ml | ||
| Tear | ≥15 μL | ≥10 μL | ||
Plant | Twigs of plants (leaf buds, tender leaves), algae | ≥300 mg | 200 mg | |
| Old leaves, roots, stems, bark of plants | ≥1 g | 500 mg | ||
| Plant buds, pollen | 100 mg | 50 mg | ||
| Plant seeds (rice/wheat seeds, etc.) fruits(apples, peaches, pears) | ≥1g | 500 mg | ||
Microorganism | Prokaryotic bacteria (E.coli, Staphylococcus aureus, etc.) fungi (yeast, etc.) | Thallus ≥50 mg cells ≥5×106 | ||
Protein solution | Complex protein solution, protein powder | ≥40 pg ≥0.5μg/μL | ≥20 pg ≥0.5μg/μL | |
Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
