Metware Biotechnology Co., Ltd.
Energy Metabolism

Energy Metabolism

energy metabolism

Energy metabolism is the process of generating energy (ATP) from nutrients such as glucose and fatty acids to generate various metabolites, including those from glycolysis pathway (EMP), tricarboxylic acid cycle (TCA), and pentose phosphate pathway (PPP). Metware Biotechnology's Energy Metabolism Assay obtains absolute quantitative detection of 68 energy related metabolites from various fluid and tissue samples. 

Technology Introduction

Energy metabolism is the process of anaerobic glycolysis and aerobic respiration from nutrients such as glucose and fatty acids for the production of energy (ATP). It is one of the most basic characteristics of life and maintains the basis of cellular function. Energy metabolism, also known as central carbon metabolism (CCM), mainly includes glycolysis pathway (EMP), tricarboxylic acid cycle (TCA), and pentose phosphate pathway (PPP). These pathways cycle through many intermediate compounds to generate energy. Metware Biotechnology uses QTRAP, a triple-quad LC-MS technology, to obtain absolute quantification of 68 metabolites associated with these pathways. One no longer have to guess how the cells or organism is doing, our assay will help quantifiy their energy uses and help you determine the affects of your experiment.

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Technology Features of Energy Targeted Metabolomics Service

Accurate compound identification

each metabolite has been developed using external standards;

Wide coverage

the panel covers 68 compounds in the three major pathways; 

Ultra sensitivity

using AB QTRAP® 6500+ LC-MS/MS for detection of ng/mL levels.

Applications of Energy Targeted Metabolomics Service

Applications: Biomarker Screening

Build cohorts, do screening of differential metabolites, build diagnostic models and performance validation markers.

Applications: Functional Studies

Do metabolite modulation and perform functional phenotype validation based on the screened target metabolite species.


Applications: Mechanism Study

Establish disease animal models and perform functional mechanism validation through multiple pathways.

Energy Targeted Metabolism Services FAQs

Which pathways are involved in energy metabolism?

It mainly contains compounds from the glycolytic pathway (EMP), the tricarboxylic acid cycle (TCA), and the pentose phosphate pathway (PPP).


Is Energy Metabolite Assay absolute quantitative or relative quantitative?

Metware Biotechnology's Energy Metabolism Assay is an absolute quantitative method. Each substance is developed by an external standard, and the absolute quantitative value is calculated according to the standard curve.


List of Metabolites

Compounds

CAS No

Class

Pyruvic acid

127-17-3

Organic acid And Its derivatives

Serine

56-45-1

Amino acids

L-Glutamic acid

56-86-0

Amino acids

Adenine

73-24-5

Nucleotide and Its metabolomics

Inosine

58-63-9

Nucleotide and Its metabolomics

3-phenyllactic acid

828-01-3

Organic acid And Its derivatives

Lactate

50-21-5

Organic acid And Its derivatives

ADP

58-64-0

Nucleotide and Its metabolomics

Uracil

66-22-8

Nucleotide and Its metabolomics

Guanosine

118-00-3

Nucleotide and Its metabolomics

c-di-AMP

54447-84-6

Nucleotide and Its metabolomics

Cyclic-AMP

60-92-4

Nucleotide and Its metabolomics

Fructose 1,6-bisphosphate

34693-15-7

Phosphate sugars

NADPH

53-57-6

Carbohydrate metabolomics



Project Workflow of Energy Targeted Metabolomics Service

Project Workflow of Energy Targeted Metabolomics Service

Sample Requirements of Energy Targeted Metabolomics Service

ProductsSample Requirements
Serum/plasma100ul/sample, snap-frozen in liquid nitrogen and stored at -80°C. Ship on dry ice. Avoid thawing. 
Tissue100mg/sample, snap-frozen in liquid nitrogen and stored at -80°C. Ship on dry ice.
Cells≥1×106/sample, snap-frozen in liquid nitrogen and stored at -80°C. Ship on dry ice.
Feces and intestinal contents (wet weight)200 mg/sample; snap-frozen in liquid nitrogen and stored at -80°C. Ship on dry ice.
For other sample types, please get in touch with us.
Note: For all samples, please avoid repeated freezing and thawing.


Energy Target Metabolism Services Case Study

S1P defects cause a new entity of cataract, alopecia, oral mucosal disorder, and psoriasis-like syndrome

Journal: EMBO Molecular Medicine; IF:10.624;

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Reference:

Chen F, Ni C, Wang X. et al. S1P defects cause a new entity of cataract, alopecia, oral mucosal disorder, and psoriasis-like syndrome. EMBO Mol Med. 2022 May 9;14(5):e14904. doi: 10.15252/emmm.202114904. Epub 2022 Apr 1. PMID: 35362222; PMCID: PMC9081911.


WHAT'S NEXT IN OMICS: THE METABOLOME
WHAT'S NEXT IN OMICS: THE METABOLOME

Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.