( Suitable for Untargeted Metabolomics/TM Widely-Targeted Metabolomics/Quantitative Lipidomics/Energy Metabolism)
Sample type | sample | Recommended sample size | Minimum sample size | Biological replication |
liquid | Plasma, serum, hemolymph, milk, egg white | 100 μL | 20μL | human>30 animal>6 |
Cerebrospinal fluid, tear fluid, interstitial fluid, uterine fluid, pancreatic fluid and bile, pleural effusion, follicular fluid, culture medium | 100 μL | 20μL | ||
Seminal plasma, amniotic fluid, prostate fluid, rumen fluid, respiratory condensate, gastric lavage fluid, alveolar lavage fluid, urine, sweat, corpse fluid, saliva, sputum | 500 μL | 100 μL | ||
tissue | Animal tissue, placenta, thrombus, fish skin, mycelium, nematode | 100 mg | 20 mg | |
Whole body, aircraft (wings), pupae | 500 mg | 20 mg | ||
Zebrafish organs, insect organs | 20 | 10 | ||
cell | Adherent cells | 1*10^6 | 5*10^5 | |
Escherichia coli and other microorganisms | 1*10^10 | 5*10^8 | ||
feces | Stool samples, intestinal contents | 200 mg (Wet weight) | 50 mg (Wet weight) | |
( Suitable for Widely-Targeted Metabolomics for Plants/Flavonoids Metabolomics )
Sample Type | Recommended sample size | Minimum sample size | Dry or not |
Fresh Tissue Sample: root, stem, leaf, flower, fruit, seed | 600mg | 300mg | Yes |
Dry Sample | 50mg | 30mg | Yes |
Root secretion | 10ml | 3ml | Yes |
Wine, Fermented Broth | 5ml | 3ml | Yes |
tissue fluid, extracting solution | 500μl | 200μl | No |
juice | 5ml | 3ml | Yes |
oil, essential oil | 500μl | 200μl | No |
honey | 500μl | 200μl | No |
Special tissue(trichome) | 50mg | 15mg | No |
Regarding biological replicates: We recommend collecting from 3 or more individuals for each biological replicates and we recommend having a minimum of 3 biological replicates for each group of samples. For example: mix leaves from 3 individual plants as one replicate. Repeat for 2 more replicates (6 more individual plants) to obtain 3 biological replicates for one experimental group (e.g. Control). Do the same from 9 individual plants for the second experimental group (e.g. Treatment).
Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.