Metware Biotechnology Co., Ltd.
Metware Cloud Platform

Sample Requirements

Animal tissue and fluids

( Suitable for Untargeted Metabolomics/TM Widely-Targeted Metabolomics/Quantitative Lipidomics/Energy Metabolism)

Sample type

sample

Recommended sample

Minimum sample

Biological replication

liquid

Plasma, serum, hemolymph, milk, egg white

100 μL

20μL

human>30

animal>8


Cerebrospinal fluid, tear fluid, interstitial fluid, uterine fluid, pancreatic fluid and bile, pleural effusion, follicular fluid, culture medium

100 μL

20μL

Seminal plasma, amniotic fluid, prostate fluid, rumen fluid, respiratory condensate, gastric lavage fluid, alveolar lavage fluid, urine, sweat, corpse fluid, saliva, sputum

500 μL

100 μL

tissue

Animal tissue, placenta, thrombus, fish skin, mycelium, nematode

100 mg

20 mg

Whole body, aircraft (wings), pupae

500 mg

20 mg

Zebrafish organs, insect organs

20

10

cell

Adherent cells

1*10^6

5*10^5

Escherichia coli and other microorganisms

1*10^10

5*10^8

feces

Feces,iIntestinal contents

200 mg

(Wet weight)

50 mg

(Wet weight)


Plants

Suitable for Widely-Targeted Metabolomics for Plants/Flavonoids Metabolomics )


Sample Type

Recommended Sample

Minimum SampleDry or not

Fresh Tissue Sample: root、stem、leaf、flower、fruit

600mg

300mgYes

Dry Sample

50mg30mgYes

Root secretion

10ml

3ml

Yes

Wine,Fermented Broth

5ml

3ml

Yes

tissue fluid、extracting solution

500μl200μl

No

juice

5ml

3ml

Yes

oil、essential oil

500μl200μl

No

honey

500μl200μl

No

Special tissue(trichome)

50mg

15mg

No


Regarding biological replicates: We recommend collecting from 3 or more individuals for each biological replicates and we recommend having a minimum of 3 biological replicates for each group of samples. For example: mix leaves from 3 individual plants as one replicate. Repeat for 2 more replicates (6 more individual plants) to obtain 3 biological replicates for one experimental group (e.g. Control). Do the same from 9 individual plants for the second experimental group (e.g. Treatment).


WHAT'S NEXT IN OMICS: THE METABOLOME
WHAT'S NEXT IN OMICS: THE METABOLOME

Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.