Chromatography Techniques Series

Chromatography is one of the most foundational and versatile techniques in analytical chemistry, enabling the separation, identification, and quantification of components in complex mixtures. Since its invention in the early 20th century, chromatography has undergone continuous evolution—from classical column methods to advanced high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC). Today, it is indispensable across fields such as pharmaceuticals, biotechnology, environmental science, food safety, and proteomics. As a front-end technique often coupled with mass spectrometry, chromatography plays a critical role in improving selectivity, sensitivity, and reproducibility in modern analytical workflows.

This blog collection offers a comprehensive overview of chromatography technologies, with a focus on both theory and practical application. Topics include an all-in-one primer on chromatographic principles, in-depth comparisons of LC, HPLC, and UHPLC, and strategic guidance on choosing between analytical, semi-preparative, and preparative HPLC based on scale and precision. We also dive into the characteristics and applications of core column types, and provide a comparative guide to popular separation techniques—including RPLC, HILIC, IEX, GPC/SEC, and affinity chromatography. Whether you're a beginner seeking foundational knowledge or an experienced user optimizing your workflows, this series delivers valuable insights to elevate your chromatography expertise.

For in-depth insights on each topic, please explore the blogs below:

1. Mastering Chromatography: Everything You Need to Know

2. Chromatographic Columns: The Backbone of Analytical Chemistry

3. LC vs. HPLC vs. UHPLC: Tracing the Evolution of Chromatographic Techniques

4. Analytical vs. Semi-Preparative vs. Preparative HPLC: A Strategic Guide to Precision, Scale, and Efficiency

5. Comparative Guide to Protein Separation and Detection Chromatography: RPLC vs. HILIC vs. IEX vs. GPC/SEC vs. AC