DIA quantitative proteomics technology utilizes the unique dual TIMS design of the timsTOF series mass spectrometer and the diaPASEF® acquisition mode to achieve qualitative and quantitative protein analysis. The diaPASEF acquisition mode combines the data-independent acquisition (DIA) mode with PASEF (Parallel Accumulation–Serial Fragmentation) technology. In the diaPASEF acquisition mode, the mass spectrum scan range is divided into multiple windows based on the mass-to-charge ratio (m/z) information of all precursor ions in the MS1 spectra. Subsequently, all precursor ions in each window are sequentially fragmented in the MS2 spectra, recording their retention time, mass-to-charge ratio, ion intensity, and ion mobility. Finally, protein qualitative and quantitative analysis is conducted based on this information.

DIA proteomics combines the advantages of 4D proteomics and DIA technology while overcoming some of their inherent limitations. Based on the Bruker timsTOF HT platform, DIA proteomics enhances ion utilization and identification accuracy, significantly improves the coverage, sensitivity, and throughput of protein detection technology with less sample input and faster scanning speed.