Quantitative Lipidomics

Lipidomics assay is the systematic analysis of lipidomics and lipid detection in organisms, tissues or cells and the molecules with which they interact. Quantitative Lipidomics is a high-throughput targeted lipidomics approach. Our lipid quantification simultaneously identifies and quantifies with semiquantification of over 4000 lipids.

Technology Introduction

Lipidomics refers to the study of the structure, function, content changes, and interactions of lipids in organisms to reveal the regulatory role of lipid metabolism on the physiological and pathological state of the organism. Quantitative Lipidomics is a high-throughput targeted approach that simultaneously identifies and quantifies with semiquantification of over 4000 lipids. Metware Biotechnology has developed a professional method for the detection of lipids, covering 51 kinds, such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG.


Technical Features of Quantitative Lipidomics Service

High Throughput
4000+ lipids can be detected at one time in the lipid detection.
Wide Coverage
Simultaneous detection of more than 51 types of lipids such as CE, Cer, HexCer, SM, FA, CAR, Eicosanoid, PC, LPC, PE, LPE, PG, LPG, PS, LPS, PI, TG, DG, etc.
High Reproducibility
Better reproducibility of multiple testing results, with intra-batch correction for large sample volumes.
High Sensitivity
The use of highly sensitive mass spectrometer AB SCIEX6500+, detection down to pg level.
High Accuracy
Absolute quantitative analysis of lipids and lipid quantification by internal standard method with more accurate results (standard + isotope internal standard).

Applications of Quantitative Lipidomics Service

  • Clinical disease research: applied to various types of disease research such as cardiovascular, tumor, immune, endocrine, gastrointestinal, and neurological.
  • Screening of Biomarkers: by studying the differences in lipid profiles between different groups, screening of Biomarkers can be used for research on disease diagnosis, disease staging/staging, prognosis determination, etc.
  • Mechanism studies: e.g. mechanism studies of lipid effects on growth, development, energy metabolism, signal transduction processes, and diseases.
  • Pharmaceutical therapy: e.g. polyketide anti-microbial, anti-parasitic and anti-tumor drugs.
  • Multi-omics joint research: combined with other histologies to study the physiological and pathological effects of the organism, such as the elucidation of regulatory genes of important lipids that affect disease health and the mechanisms of regulation.
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Demo Final Report Of Quantitative Lipidomics

Demo Final Report Of Quantitative Lipidomics
List of Metabolites
Number of Lipids
Class I Class II Number
Fatty acyls(FA) CAR, FFA,  Eicosanoid, FAHFA 270
Glycerolipids(GL) DG, DG-O, MG, TG, TG-O, MGDG, DGDG 1015
Glycerophospholipids(GP) LPC, LPC-O, LPE, LPE-P, LPG, LPS, PC, PC-O, PE, PE-P, PE-O, PG, PS, LPI, PI, LPA, PA, PMeOH, BMP, HMBP, LNAPE 1800
Sphingolipids(SL) SPH, CerP, HexCer, SM, Cer, Cert 828
Sterol lipids(ST) Cho, CE, BA, CASE 122
Prenol lipids(PR) CoQ 3
Total 4000+


Project Workflow of Quantitative Lipidomics Service

Our Expertise

Lipid Identification

We leverage an extensive collection of 200+ purified chemical standards encompassing diverse lipid classes, laying the groundwork for precise lipid identification and retention time pattern evaluation. Augmented by comprehensive references and varied lipid databases, our approach facilitates the accurate discernment of a broad spectrum of lipid compounds.

Lipid Quantitation

Employing the gold standard for metabolite quantification—mass spectrometry's multiple reaction monitoring (MRM) detection mode—we guarantee precise lipid quantification. This mode not only enhances accuracy but also enables the detection of minute quantities of lipid-like substances. For instance, in a mouse liver sample, we can detect over 1700 lipids.

High Reproducibility

The utilization of the MRM mode within mass spectrometry stands as a pivotal element in ensuring the consistent and stable detection of lipids, especially when analyzing extensive sample cohorts.


Sample Requirements of Quantitative Lipidomics Service

Sample type


Recommended sample

Minimum sample

Biological replication


Plasma, serum, hemolymph, milk, egg white

100 μL





Cerebrospinal fluid, tear fluid, interstitial fluid, uterine fluid, pancreatic fluid and bile, pleural effusion, follicular fluid, culture medium

100 μL


Seminal plasma, amniotic fluid, prostate fluid, rumen fluid, respiratory condensate, gastric lavage fluid, alveolar lavage fluid, urine, sweat, corpse fluid, saliva, sputum

500 μL

100 μL


Animal tissue, placenta, thrombus, fish skin, mycelium, nematode

100 mg

20 mg

Whole body, aircraft (wings), pupae

500 mg

20 mg

Zebrafish organs, insect organs




Cultured cells



Escherichia coli and other microorganisms




Feces,iIntestinal contents

200 mg 

(Wet weight)

50 mg

(Wet weight)


Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
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