Blood Quantitative Proteomics is a specialized method developed specifically for blood samples. Blood contains a diverse range of proteins with significant differences in abundance, with high-abundance proteins dominating. In conventional proteomics, signals from low-abundance proteins relevant to many disease indicators in the blood are often overshadowed by signals from high-abundance proteins, resulting in fewer detected proteins. Our quantitative blood proteomics approach first enriches the middle-to-low abundance proteins in the blood using nanomagnetic beads. Subsequently, the samples are analyzed using the diaPASEF scanning mode, which offers high throughput, increased protein detection, and improved depth of analysis.