+1(781)975-1541
support-global@metwarebio.com

Phosphorylation Proteomics

Phosphorylation Proteomics

Highly specific enrichment material Ti-IMAC for comprehensive coverage of modified proteins.
High-sensitivity and high-resolution mass spectrometry platform.
High-throughput 4D protein profiling detection technology.

Technology Introduction

Protein phosphorylation refers to the process by which a protein transfers the phosphate group on ATP or GTP to a specific site (amino acid residue Ser, Tyr, Thr) on a protein under the catalysis of phosphorylase. Phosphorylation is a widely occurring post-translational modification, with over 30% of cellular proteins undergoing phosphorylation. Therefore, phosphorylation is the most fundamental, widespread, and important mechanism for regulating and controlling protein activity and function. Phosphorylation is involved in various physiological and pathological processes, regulating cellular proliferation, development, differentiation, apoptosis, and other life activities, and is widely applied in research fields such as signal transduction pathways, apoptosis, development and differentiation, and cancer mechanisms.

Phosphorylation proteomics aims to identifying phosphorylated proteins and peptides in diverse samples, accurately localizing and quantifying phosphorylation sites, and ultimately elucidating their biological functions. This methodology begins with protein sample digestion into peptide mixtures, followed by liquid chromatography to separate digested peptide components and reduce sample complexity. Subsequently, phosphorylation peptides are enriched using high-quality phosphorylation modification antibodies and biological materials. Finally, mass spectrometry technology with DDA (data-dependent acquisition) mode is employed for analysis and quantification.

tech

Applications of Phosphorylation Proteomics

Medical Research
Disease Mechanisms, Molecular Diagnosis, Biomarker Development, Drug Targets.
Animal Research
Reproductive Development, Disease Mechanisms, Nutrient Metabolism, Animal Toxicology.
Plant Research
Reproductive Development, Abiotic Stress Response, Disease Resistance, Crop Improvement.
Microbiology
Pathogenic Mechanisms, Drug Resistance, Stress-Related Proteins Screening, Environmental Impact Mechanisms.
Project Workflow
1
Biological Sample
2
Total Proteins
3
Total Peptides
4
Phosphopeptides
5
RAW Data
6
Bioinformation Report

Sample Requirements

Sample Type Samples Recommended Sample Size Minimum
Sample Size
Human/Animal Tissue Normal tissues (heart, liver, spleen, lungs, intestines, kidneys, etc.) 50mg 30mg
Fatty tissue 1g 500mg
Brain tissue 100mg 50mg
Bone 1g 500mg
Hair 1g 500mg
Plant Tissue Young tissue (young leaf, seedling, petal, etc.) 500mg 200mg
Mature tissue (root, stem, fruit, pericarp, etc.) 2g 1.5g
Joint fluid, Lymph fluid 1ml 500μL
Cerebrospinal fluid 1ml 500μL
Amniotic fluid 5ml 2ml
Urine 50mL 20mL
Fungi 1g 500mg
Cells Primary Cells 2×10^7 1×10^7
Transmissible cells 1×10^7 5×10^6
Sperm, Platelets 5×10^7 2×10^7
Protein Protein 1000μg 500μg
Biological duplicates: A minimum of 3 replicates is required; 3-6 replicates for animal samples; 6-10 for clinical samples.

 

WHAT'S NEXT IN OMICS: THE METABOLOME

Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
Name can't be empty
Email error!
Message can't be empty
CONTACT FOR DEMO
+1(781)975-1541
LET'S STAY IN TOUCH
submit
Copyright © Metware Biotechnology Inc. All Rights Reserved.
support-global@metwarebio.com +1(781)975-1541
8A Henshaw Street, Woburn, MA 01801
Contact Us Now
Name can't be empty
Email error!
Message can't be empty