MetwareBio Lipidomics Services
MetwareBio Lipidomics Services
What is Lipidomics and Why It Matters in Lipid Analysis
Lipidomics is the large-scale analysis of lipids, providing direct insights into their composition, abundance, and structural diversity in biological samples. Lipids are dynamic biomolecules that play indispensable roles in energy storage, cellular membrane integrity, and intricate signaling pathways. They are increasingly recognized as pivotal players in metabolic disorders, cancer progression, neurodegenerative diseases, and immune responses. Lipidomics enables researchers to comprehensively characterize lipid species, monitor their fluctuations under different conditions, and decode their contributions to health and disease. Through advanced lipid profiling and quantitative mapping of lipid alterations, lipidomics research provides powerful avenues for biomarker discovery, therapeutic target identification, and a deeper understanding of molecular mechanisms underlying complex biological systems.
MetwareBio offers end-to-end lipidomics services specifically designed to meet the evolving needs of modern biomedical and agricultural research. Leveraging a vast proprietary lipid database, our quantitative lipidomics workflows enable confident identification of diverse lipid classes, while employing internal standards and MRM acquisition modes to achieve precise, reproducible quantitation. We provide specialized quantitative lipidomics solutions tailored for medical and animal studies, alongside dedicated plant lipidomics workflows that address the unique complexity of plant lipid compositions. From comprehensive lipid analysis services to expert consultation at every stage, MetwareBio equips your research with robust, reliable data that accelerates discovery and drives meaningful scientific insights.
Discover MetwareBio Lipidomics Services
Lipidomics Service General Knowledge
Mass-spectrometry based lipidomics is one of the main techniques to study different species of lipids and lipidomics is a very active branch of omics research. Global lipid profiling as well as targeted metabolomics lipidomics can be achieved using LC-MS followed by lipid annotation with specialized lipidomics database. At Metware, a reliable metabolomics service providers, we have developed a specialized lipid spectra database that includes those in public lipidomics database as well as over 4000 in-house lipidomics data using chemical standards and AI prediction.
Lipidomics Analysis Services Equipment
Metware Biotechnology offers detection and quantification of up to 4000 lipid compounds in our Quantitative Lipidomics analysis services. This process uses external standards and MRM scanning mode to provide accurate identification and absolute quantification of the lipids in the sample. Our clients have published their results in Gastroenterology, Nature Communications, and Metabolism: Clinical and Experimental. Our panel of 3000 lipids spans 6 major classes of lipids and is sensitive enough to distinguish different carbon-chain lengths and saturation.
Lipidomics Service Benefits
Comprehensive Coverage
Simultaneous detection of over 40 types of lipids.
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High Sensitivity
Using ultra-sensitive AB Sciex 6500+ instrument to detect lipids at pg level.
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Accurate Annotation And Quantification
Annotation using RT + Q1/Q3 data of multiple ions and mapping to a curated lipids database.
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Stringent QC
9 major QC points
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Why Choose MetwareBio for Lipdomics Services
Extensive Proprietary Databases
Our quantitative lipidomics platforms are powered by large, self-developed libraries—covering over 4,000 lipids for animal studies and over 1,700 lipids for plant analyses—ensuring comprehensive and confident lipid identification.
Broad Coverage Across Major Lipid Classes
We deliver in-depth lipid profiling across six major lipid categories, including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, and prenol lipids, enabling a holistic view of lipid metabolism.
Accurate Identification and Quantification
Lipids are accurately identified using our proprietary databases and precisely quantified through MRM acquisition modes coupled with internal standards, guaranteeing robust semi-quantitative results.
Exceptional Sensitivity and Throughput
Leveraging the ultra-sensitive AB Sciex 6500+ platform, our system achieves detection limits down to picogram levels, enabling precise analysis of low-abundance lipids while maintaining high sample throughput.
Superior Stability and Reproducibility
Rigorous QA/QC workflows ensure outstanding data stability and repeatability, providing confidence in your results across batches and studies.
Specialized Extraction and Separation Techniques
We employ MTBE-based extraction methods and advanced C30 chromatographic separation to maximize lipid recovery and resolution, supporting high-quality downstream analysis.
MetwareBio Lipid Profiling WorkFlow
MetwareBio’s lipidomics workflow is comprehensively optimized to deliver robust, high-quality results at every stage. Starting with carefully tailored sample preparation, we employ MTBE-based extraction to efficiently isolate a broad range of lipid molecules. The extracted lipids are then separated using advanced C30 chromatography, enhancing resolution and coverage of diverse lipid classes. Detection and quantification are performed on state-of-the-art LC-MS/MS systems in MRM mode, ensuring precise, reproducible measurement of lipid abundances. Finally, our powerful bioinformatics pipelines integrate identification and quantification data to generate clear, insightful interpretations, providing a strong foundation for data-driven lipidomics research.
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Sample Preparation
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Lipid Extraction
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LC-MS/MS Detection
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Identification&
Quantification
Quantification
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Data Analysis
Comprehensive Lipid Analysis Results with Bioinformatics Visualization
Our lipidomics data analysis workflow integrates rigorous quality control and advanced statistical interpretation to extract meaningful biological insights. It begins with QC assessments, including TIC overlays, correlation analysis, CV distribution, and PCA to ensure data reliability. We then perform comprehensive lipid composition, chain length, and unsaturation analyses, followed by comparative PCA and OPLS-DA to explore group differences. Differential lipid screening is visualized through volcano plots, scatter plots, radar charts, and correlation heatmaps. Additional K-means clustering, KEGG pathway enrichment, and ROC curve analysis further reveal key lipid alterations and potential biomarkers, delivering a complete, insightful visualization of your lipidomics results. Contact Us for Demo
Lipid Dynamic Distribution
Chain Length Analysis
Chain Unsaturation Analysis
PCA Analysis
Volcanic Plot
Heatmap
Correlation Analysis
Chord Diagram
K-Means Diagram
KEGG pathway
KEGG Enrichment
ROC Curve
Lipidomics Services Overview
Mass spectrometry-based lipidomics is a core technology for systematically analyzing diverse lipid species and is one of the most dynamic fields in modern omics research. Both global lipid profiling and targeted lipidomics studies rely on high-performance LC-MS workflows, followed by precise lipid annotation using specialized lipid databases. As a leading metabolomics and lipidomics service provider, MetwareBio has built an extensive lipid spectral library that combines public lipidomics data with over 4,000 proprietary spectra generated from chemical standards and advanced AI predictions. This comprehensive database strengthens the accuracy of lipid identification and quantification, ensuring reliable lipid analysis results that drive high-impact lipidomics research.
Technical FAQs on Lipidomics Services
1. What is lipidomics and why is it important in biomedical research?
Lipidomics is the large-scale study of lipids within biological systems, enabling detailed lipid profiling and quantification. By revealing changes in lipid composition and metabolism, lipidomics plays a critical role in understanding disease mechanisms, discovering biomarkers, and developing targeted therapies.
2. What lipid classes can your lipidomics services detect?
Our lipidomics services cover six major lipid categories—fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, and prenol lipids—spanning 51 subclasses. This includes many lipids of high research interest such as CE, Cer, HexCer, SM, FA, CAR, eicosanoids, PC, LPC, PE, PI, TG, and DG, ensuring comprehensive and detailed lipid profiling for your studies.
3. Is your quantitative lipidomics targeted lipidomics or untargeted lipidomics?
Our quantitative lipidomics is not strictly targeted. It uses our proprietary lipid database for identification and combines internal standards with MRM acquisition to deliver semi-quantitative data, including absolute concentrations. This means it does not fall under typical untargeted lipidomics. (Our untargeted lipidomics service will be available soon.)
4. How does quantitative lipidomics differ from untargeted metabolomics for lipid analysis?
Untargeted metabolomics is designed to detect a broad range of metabolites and is optimized for small polar compounds, so it captures only a portion of lipids. In contrast, our quantitative lipidomics is specifically developed for lipid profiling, using MTBE extraction, C30 chromatography, and MRM methods to maximize lipid coverage and achieve high-sensitivity semi-quantitation.
5. What lipid classes can your lipidomics services detect?
Our lipidomics platform covers six major lipid categories across 51 subclasses, including many lipids critical in disease and metabolic research such as CE, Cer, HexCer, SM, FA, CAR, eicosanoids, PC, LPC, PE, PI, TG, and DG, ensuring comprehensive lipid profiling.
6. Can your lipidomics analysis differentiate lipid saturation levels?
Yes. Our data analysis includes chain length and saturation assessments, providing clear insights into the distribution of saturated, monounsaturated, and polyunsaturated lipids. However, current technology does not resolve positional double-bond isomers within the same lipid species.
7. How many lipids can you typically detect in biological samples?
From our extensive project experience, we generally detect 1,100+ lipids in animal or medical samples such as tissues and cells, and 700+ lipids in typical plant samples.
8. Can you analyze sebum collected by skin tapes or patches?
Yes. We have significant experience with skin tape and patch-collected sebum samples. Usually, 1–2 skin tapes or patches are sufficient for our lipidomics analysis.
Applications of Lipidomics Services
Biomedical Research and Clinical Studies
Lipidomics plays a vital role in understanding disease mechanisms, particularly in areas such as metabolic disorders, cardiovascular disease, cancer, and neurodegeneration. Through precise lipid profiling and differential analysis, researchers can identify disease-specific lipid biomarkers, uncover metabolic dysregulation, and evaluate therapeutic responses—making quantitative lipidomics a valuable tool for translational and clinical research.
Animal Health and Nutrition
In animal studies, lipidomics analysis helps reveal the impact of diet, stress, genetics, or pharmacological interventions on lipid metabolism. It is widely used in veterinary science, livestock breeding, and nutritional evaluations. By profiling tissue-specific or systemic lipid changes, lipidomics supports improved animal health and optimized feed formulations.
Plant Science and Crop Research
Plant lipidomics enables in-depth investigation of lipid composition and dynamics in crops, model plants, and stress-response studies. It aids in understanding membrane remodeling, lipid signaling, and energy storage under environmental conditions such as drought, cold, and pathogen attack. Our plant lipidomics services support research in plant physiology, genetics, and agricultural biotechnology.
Microbial Studies and Biotechnology
Lipidomics is increasingly applied to explore lipid metabolism in microbes, including bacteria, fungi, and microalgae. It helps elucidate membrane lipid composition related to stress adaptation or antibiotic resistance, characterize bioactive lipid secondary metabolites, and optimize microbial production of industrially valuable lipids like PUFAs or biodiesel precursors. In pathogenic microorganisms, lipid profiling also provides insights into virulence mechanisms and host–pathogen interactions.
Applications of Metabolomics Services
Next-Generation Omics Solutions:
Proteomics & Metabolomics
Have a project in mind? Tell us about your research, and our team will design a customized proteomics or metabolomics plan to support your goals.
Ready to get started? Submit your inquiry or contact us at support-global@metwarebio.com.
Ready to get started? Submit your inquiry or contact us at support-global@metwarebio.com.
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