Proteomics analysis of clinical or rare samples is often limited by insufficient protein input. Traditional workflows involve multiple steps—protein extraction, digestion, and cleanup—that can lead to significant sample loss and poor recovery, making standard DIA-based proteomics unsuitable for low-input scenarios. This presents a major barrier for studies using biopsy tissues, sorted cells, laser-microdissected regions, or other precious samples where only nanogram to low-microgram quantities are available.

MetwareBio's Low-Input (Micro-DIA) Quantitative Proteomics Service overcomes this limitation by integrating the SISPROT (Single-Tube Sample Preparation for Proteomics) technology, which performs protein extraction and enzymatic digestion in a streamlined, single-tube workflow. This minimizes handling steps and sample loss while preserving protein recovery and digestion efficiency. Combined with diaPASEF® acquisition on the Bruker timsTOF platform, this workflow delivers deep proteome coverage, high sensitivity, and excellent reproducibility, even from microgram-scale inputs. The micro-DIA platform is ideally suited for low-input clinical studies, microenvironment-specific proteomics, and high-throughput small-sample investigations, expanding the reach of proteomics into challenging yet highly valuable biological systems.