PRM Targeted Proteomics
PRM Targeted Proteomics
Technology Overview: Parallel Reaction Monitoring (PRM) for Targeted Proteomics
MetwareBio’s PRM-Based Targeted Proteomics Service is built on the Bruker timsTOF platform, equipped with Trapped Ion Mobility Spectrometry (TIMS) and the advanced prmPASEF acquisition mode. This powerful setup ensures highly efficient ion utilization, exceptional sensitivity, and accurate quantification of target peptides with outstanding reproducibility. With robust performance and high-throughput capability, the service is ideally suited for biomarker validation, drug target verification, pathway-specific analysis, and translational or clinical proteomics applications.
prm-PASEF workflow for targeted proteomics experiments (Lesur et al., 2021)
Why Choose Our PRM Proteomics Service
Applications of PRM-Based Targeted Proteomics
PRM is widely used in clinical proteomics for the sensitive and reproducible quantification of disease-associated proteins. It enables biomarker validation, therapeutic target verification, and longitudinal monitoring of protein expression in plasma, tissue, or FFPE samples—supporting diagnostic and precision medicine applications.
In preclinical and translational drug studies, PRM offers high-precision quantification of drug targets, pharmacodynamic markers, and proteins involved in mechanisms of action or off-target effects, facilitating informed decision-making during compound screening and evaluation.
PRM supports targeted protein quantification in model organisms and agricultural species, helping researchers investigate developmental pathways, stress responses, and hormone signaling. In crops and livestock, PRM aids in functional protein validation and trait-related biomarker discovery.
In microbial systems and environmental samples, PRM enables precise measurement of regulatory enzymes, virulence factors, and stress-related proteins, supporting studies in pathogenesis, bioremediation, and host–microbe interactions.
PRM Proteomics Sample Requirements
| Sample Type | Samples | Recommended Sample Size | Minimum Sample Size |
| Human/Animal Tissue | Normal tissues (heart, liver, spleen, lungs, intestines, kidneys, etc.) | 50mg | 5mg |
| Fatty tissue | 200mg | 100mg | |
| Brain tissue | 50mg | 5mg | |
| Bone | 1g | 200mg | |
| Hair | 500mg | 200mg | |
| Skin | 200mg | 100mg | |
| Plant Tissue | Young tissue (young leaf, seedling, petal, etc.) | 200mg | 100mg |
| Mature tissue (root, stem, fruit, pericarp, etc.) | 1g | 500mg | |
| Pollen | 40mg | 15mg | |
| Liquid Samples | Serum/Plasma (without removing high abundance proteins) | 20μL | 5μL |
| Serum/Plasma (remove high abundance proteins) | 200μL | 100μL | |
| Joint fluid, Lymph fluid | 200μL | 100μL | |
| Aqueous humor, Vitreous body | 300μL | 200μL | |
| Cerebrospinal fluid | 200μL | 100μL | |
| Ascites, Follicular fluid | 100μL | 50μL | |
| Alveolar lavage fluid (BALF) | 1ml | 500μL | |
| Amniotic fluid | 1ml | 500μL | |
| Milk | 20μL | 5μL | |
| Urine | 10mL | 5mL | |
| Saliva (mammals) | 1ml | 500μL | |
| Fermentation broth, Bacterial solution | 10ml | 5ml | |
| Cellular supernatant | 25mL | 10ml | |
| Exosome (sediment) | 25μl | 15μL | |
| Microorganisms | Bacteria | 200mg | 100mg |
| Fungi | 300mg | 150mg | |
| Cells | Primary Cells | 3×10^6 | 1×10^6 |
| Transmissible cells | 2×10^6 | 1×10^6 | |
| Sperm, Platelets | 2×10^7 | 1×10^7 | |
| Protein | Protein | 100μg | 50μg |
Frequently Asked Questions about PRM Proteomics
PRM (Parallel Reaction Monitoring) is a targeted mass spectrometry technique optimized for precise quantification of predefined peptides or proteins. Unlike DIA or DDA, which aim for broad proteome coverage, PRM provides higher selectivity and quantitative accuracy, making it ideal for validating candidate biomarkers or pathway-specific proteins.
To ensure accurate detection and quantification, we use DIA-based acquisition (diaPASEF) to generate high-quality spectral libraries. This approach ensures comprehensive peptide coverage, reproducible fragment ion patterns, and robust assay development for PRM.
Our PRM workflow typically supports dozens to hundreds of peptides per run, depending on the complexity of the sample and retention time scheduling. We tailor assay design to balance multiplexing depth with signal quality.
Yes. PRM is particularly effective for validating candidate proteins identified in discovery-phase studies, including DIA or DDA. It offers higher quantitative precision and consistency across samples, making it ideal for verification and translational studies.
Yes. PRM is highly compatible with IP-based protein enrichment workflows. We recommend using at least 20 μL of magnetic bead pellet or 50 μL of IP eluate for optimal detection and quantification of target proteins.
Yes. We can analyze gel-excised protein bands or regions following SDS-PAGE separation. To assess feasibility, we require an image of the gel to evaluate target protein abundance and background complexity before proceeding.
Reference
Lesur, A., Schmit, P. O., Bernardin, F., Letellier, E., Brehmer, S., Decker, J., & Dittmar, G. (2021). Highly Multiplexed Targeted Proteomics Acquisition on a TIMS-QTOF. Analytical chemistry, 93(3), 1383–1392.https://doi.org/10.1021/acs.analchem.0c03180
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