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One-Carbon Metabolism Targeted Metabolomics

Covering 28 key metabolites in the one-carbon metabolism pathway
Achieving absolute quantification with both external and internal standards
Detecting at ng/mL-level sensitivity using the LC–MS/MS platform
Ensuring data reliability through rigorous quality control protocols

Technology Introduction to One-Carbon Metabolism Targeted Metabolomics

One-carbon metabolism is a vital biochemical pathway that plays a key role in numerous essential cellular processes, including nucleotide synthesis, DNA methylation, and energy production. It is integral to various physiological functions such as cell growth, tissue repair, and development. Disruptions in one-carbon metabolism are linked to the development of several diseases, including cancer, cardiovascular conditions, and neurodegenerative disorders. Understanding the regulation of one-carbon metabolism and accurately measuring its metabolites is critical for advancing research and developing targeted therapeutic interventions.
MetwareBio’s one-carbon metabolism-targeted metabolomics service utilizes state-of-the-art LC-MS (liquid chromatography-mass spectrometry) technology to provide precise, reliable analysis of 28 key metabolites within the one-carbon metabolism pathway. By employing both internal and external standards, our platform ensures accurate absolute quantification of each compound, offering high sensitivity and precision. This robust approach enables in-depth insights into metabolic variations, supporting both research and clinical applications focused on understanding disease mechanisms and optimizing treatment strategies.

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Technology Superiority of One-Carbon Metabolism Targeted Metabolomics

Comprehensive Coverage
Targeting 28 key metabolites across the one-carbon metabolism pathway, including essential compounds like tetrahydrofolate (THF), homocysteine, and methionine.
Absolute Quantification
Establishing standard calibration curves for 28 metabolites (r > 0.99) with the use of 21 internal standards to ensure precise and reproducible quantification.
High Sensitivity
Leveraging the AB QTRAP® 6500+ LC–MS/MS platform for precise detection at ng/mL concentrations, ensuring sensitivity for trace metabolites.
Rigorous Quality Control
Implementing stringent QC protocols—including blanks, solvent controls, and mixed standards—to ensure data accuracy, reliability, and consistency.

Applications of One-Carbon Metabolism Targeted Metabolomics

Metabolic Dysregulation and Disease Mechanisms
Biomarker Discovery and Validation

One-carbon metabolism metabolites are crucial for cellular processes such as methylation and nucleotide synthesis. Our platform helps researchers identify and validate novel biomarkers associated with disease progression, therapeutic response, and early disease detection. This application is especially valuable in clinical research, where precise biomarker identification can improve diagnosis and treatment strategies.

Nutritional and Environmental Metabolism

The regulation of one-carbon metabolism is sensitive to both dietary and environmental factors. Our targeted metabolomics service helps study how nutritional intake (e.g., folate, vitamin B12) and environmental exposures influence metabolic pathways. This application is essential for research into personalized nutrition, dietary recommendations, and the impact of environmental factors on metabolic health.

Epigenetics and Gene Expression Regulation

One-carbon metabolism is intricately linked to epigenetic processes such as DNA methylation, influencing gene expression. Our technology supports research on how one-carbon metabolites affect epigenetic regulation, providing insights into the molecular mechanisms of gene expression and their role in development, aging, and disease.

List of One-Carbon Metabolism Metabolites (28 Absolute-Quantified Compounds)
Index Compound Abbr. CAS No.
1 Folinic acid 5-FTHF 58-05-9
2 5-Methyltetrahydrofolate 5-MTHF 134-35-0
3 Betaine Betaine 107-43-7
4 Creatine Creatine 57-00-1
5 Cystine Cys-Cys 56-89-3
6 Cystathionine Cysta 56-88-2
7 Dimethylglycine DMG 1118-68-9
8 Glutamate Glu 56-86-0
9 Glycine Gly 56-40-6
10 Homocystine Hcy-Hcy 462-10-2
11 Homoserine Hse 672-15-1
12 Cysteine Cys 52-90-4
13 Glutathione GSH 70-18-8
14 Homocysteine Hcy 454-29-5
15 Methionine Met 59-51-8
16 S-adenosylhomocysteine SAH 979-92-0
17 S-adenosylmethionine SAM 485-80-3
18 Sarcosine Sarcosine 107-97-1
19 Serine Ser 56-45-1
20 Threonine Thr 72-19-5
21 Tryptophan TRP 73-22-3
22 Vitamin B12 VB12 68-19-9
23 Vitamin B2 VB2 83-88-5
24 Acetylcholine ACH 51-84-3
25 Choline Choline 62-49-7
26 Dihydrofolic acid DHF 4033-27-6
27 Folate FA 59-30-3
28 Tetrahydrofolate THF 135-16-0

 

Contact us to discuss your project.

One-Carbon Metabolism Targeted Metabolomics Workflow

Sample Requirement for One-Carbon Metabolism Targeted Metabolomics

Sample Class Sample Type Recommended sample size Minimum sample size
Liquid I Plasma, Serum, Hemolymph, Whole Blood, Milk, Egg White 100 μl 20 μl
Liquid II Cerebrospinal Fluid (CSF), Interstitial Fluid (TIF), Uterine Fluid, Pancreatic Juice, Bile, Pleural Effusion, Follicular Fluid, Postmortem Fluid, Tissue Fluid, Culture Medium (liquid), Culture Supernatant, Tears, Aqueous Humor, Digestive Juices, Bone Marrow (liquid) 100 μl 50 μl
Liquid Ⅲ Seminal Plasma, Amniotic Fluid, Prostatic Fluid, Rumen Fluid, Respiratory Condensate, Gastric Lavage Fluid, Bronchoalveolar Lavage Fluid (BALF), Urine, Sweat, Saliva, Sputum 500 μl 50 μl
Tissue I Small Animal Tissues, Placenta, Blood Clot, Nematode, Zebrafish (whole fish), Bone Marrow (solid), Nail 100 mg 50 mg
Tissue II Large Animal Tissues, Whole Insect Body, Wings (of insects), Pupa, Eggs, Cartilage, Bone (solid) 500 mg 50 mg
Tissue Ⅲ Zebrafish Organs, Insect Organs, Whole Microinsect Body (e.g., Drosophila) 20 units /
Solid I Feces, Intestinal Contents, Lyophilized Fecal Powder 200 mg 50 mg
Solid II Milk Powder, Microbial Fermentation Product (solid), Culture Medium (solid), Earwax, Lyophilized Tissue Powder, Feed, Egg Yolk, Lyophilized Egg Powder 100 mg 50 mg
Solid Ⅲ Honey, Nasal Mucus, Sputum 2 g 500 mg
Solid Ⅳ Sludge, Soil 600 mg 300 mg
Cell I Adherent Cells, Animal Cell Lines 1×106 cells 5×105 cells
Cell II E. Coli, Yeast Cells 1×1010 cells 5×108 cells
Cell Ⅲ Small Amount of Fungal Mycelial Balls/Mycelium, Unicellular Algae (Cyanobacteria), Large Quantities of Bacterial Hyphae (sediment), Mucilaginous Protoplasmic Clusters (hyphae) 100 mg /
Organelle I Lysosomes, Mitochondria, Endoplasmic Reticulum 4×107 cells 1×107 cells
Organelle II Exosomes, Extracellular Vesicles 2×109 particles 1×109 particles
Special Sample I Skin Tape or Patch 2 pieces 1 piece
Special Sample II Test Strips 2 pieces 1 piece
Special Sample Ⅲ Swab 1 piece 1 piece

Next-Generation Omics Solutions:
Proteomics & Metabolomics

Have a project in mind? Tell us about your research, and our team will design a customized proteomics or metabolomics plan to support your goals.
Ready to get started? Submit your inquiry or contact us at support-global@metwarebio.com.
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