Blog

PLS-DA in Metabolomics: Principles, Workflow, Interpretation, and Best Practices

Mar 24, 2026

Learn what PLS-DA is, how it works in metabolomics, how to interpret score plots and VIP values, and how to avoid overfitting in biomarker discovery.

Spatial Metabolomics in Liver Disease Research

Mar 23, 2026

Explore how spatial metabolomics reveals liver zonation, injury patterns, MASH progression, HCC metabolism, and clinical applications in liver disease research.

Plant Hormone Signaling Through Proteomics and Phosphoproteomics

Mar 20, 2026

Learn how proteomics and phosphoproteomics reveal rapid plant hormone signaling, from receptor activation to transcription factor regulation.

Spatial Metabolomics (MSI) Metabolite Identification: From Accurate Mass to Evidence-Integrated Annotation

Mar 20, 2026

Learn how metabolite identification works in spatial metabolomics MSI, from accurate mass screening to evidence-integrated annotation using MS/MS, spatial context, and biological validation.

LC–MS/MS Proteomics Data Analysis: From Raw Files to Publication-Ready Results

Mar 17, 2026

Learn the complete LC–MS/MS proteomics data analysis workflow, from raw file conversion and peptide identification to quantification, QC, statistics, and pathway analysis.

Plant Metabolomics in Growth and Development: Metabolic and Hormonal Networks

Mar 17, 2026

Learn how plant metabolomics reveals the metabolic and hormonal networks that control growth, development, and trait formation.

Signal Peptides and Protein Secretion: Mechanisms, Prediction, and Engineering

Mar 16, 2026

Learn how signal peptides direct protein secretion, how to predict them accurately, and how to engineer them to improve recombinant protein production.

A Practical Guide to OPLS-DA: Principles, Workflow, and Result Interpretation in Omics Data Analysis

Mar 13, 2026

Learn what OPLS-DA is, how to perform it step by step, and how to interpret score plots, VIP scores, and validation results correctly in omics data analysis.

Spatial Metabolomics for Biomarker Discovery in Precision Medicine

Mar 11, 2026

Learn how spatial metabolomics reveals tissue-specific metabolic biomarkers, supports disease stratification, and advances precision medicine.

Interactome & Interactomics: Mapping Protein Interaction Networks in Biology and Medicine

Mar 10, 2026

Learn what interactomics is, how protein interaction networks are mapped, and which MS-based methods help reveal disease mechanisms, drug targets, and functional biology.

Tissue Cryosectioning Guide for Spatial Metabolomics: Best Practices and Troubleshooting

Mar 09, 2026

Learn tissue-specific cryosectioning best practices for spatial metabolomics, including embedding, temperature control, section thickness, troubleshooting, and MSI sample prep.

PTM Proteomics Analysis Workflow: Variable Modifications, Site Localization, and FDR Control

Mar 09, 2026

Learn how to improve PTM proteomics analysis with DIA workflows, smarter variable modification settings, confident site localization, and rigorous FDR control.

Secretomics: Decoding the Secreted Proteome for Extracellular Communication

Mar 06, 2026

Learn what secretomics is, how secretome analysis works, and how mass spectrometry reveals extracellular signaling proteins for biomarker discovery and drug development.

Spatial Metabolomics Ion Mode Selection: Positive vs. Negative Polarity in MALDI-MSI and DESI-MSI

Mar 06, 2026

Learn how to choose positive or negative ion mode in spatial metabolomics MSI. This guide explains polarity selection for MALDI-MSI and DESI-MSI based on metabolite chemistry and experimental goals.

Core Instruments and Platforms for Spatial Metabolomics: MALDI-MSI, DESI-MSI, and SIMS

Mar 04, 2026

Learn how MALDI-MSI, DESI-MSI, and SIMS compare for spatial metabolomics—core hardware, resolution vs. coverage trade-offs, and a practical guide to choosing the right platform.

Proteomics Data Normalization: How to Remove Batch Effects and Non-biological Bias

Mar 04, 2026

Learn how to spot, prevent, and correct batch effects in proteomics—plus a practical workflow and method-by-method guidance for reliable quant.

Endogenous vs Exogenous Metabolites in Metabolomics: Definitions, Boundary Cases, and Source Attribution

Mar 02, 2026

Learn how to distinguish endogenous vs exogenous metabolites in metabolomics, handle gray-area “boundary cases,” and apply a practical, evidence-based framework for source attribution.

Data Calibration in TMT/iTRAQ Proteomics: How to Identify and Correct Channel Interference and Ratio Compression

Feb 28, 2026

Learn how to spot and fix TMT/iTRAQ channel interference and ratio compression—with practical QC red flags and calibration strategies (internal reference and mixed-sample/reference-channel) to improve quantitative accuracy.

Metabolite Identification in LC-MS Metabolomics: Identification Principles and Confidence Levels

Feb 27, 2026

Learn how LC–MS metabolite IDs are made—what evidence (exact mass, MS/MS, RT, CCS) really proves, and how to report MSI confidence levels (1–4) correctly.

Bottom-up vs Top-down vs Middle-down Proteomics: How to Choose the Right Strategy for PTM Analysis

Feb 26, 2026

Learn how bottom-up, top-down, and middle-down proteomics differ for PTM analysis—and how to choose the right strategy for PTM site localization, proteoform context, throughput, and PTM crosstalk.